Through a mutagenesis screen you have identified several mutations in a bacterial promoter.
Below are the wild type (WT) and mutant sequences that you identified and isolated from the
screen. Describe the composition of the bacterial RNA polymerase and functions associated with
each component. Describe that predicted effect of each of the mutations on transcription.
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WT 5’-GTGAGT—18 nucleotides—TATAAT-3’
MutA 5’-TTGACA—14 nucleotides—TATAAT-3’
MutB 5’-TTGACA—17 nucleotides—TACAGT-3’