By Week 10, Day 3, consider
the following questions: assignment 1
- Explain what is meant by “indicator” organisms when
testing a water sample. What are the media used to determine the presence
of the indicator organisms? What are the morphological and biochemical
characteristics of these indicator organisms?
- Discuss the three major species of Staphylococcus.
Explain why one of them is more pathogenic compared to the other two
species. Which factor determines the pathogenesis of the organisms?
the same day, present your responses to both the questions in a one-to-two page
Word document formatted in APA style and submit it
Review Sheet Week 10
1. In what areas of the hospital must the numbers of
microorganisms in the
environment be strictly reduced to the minimum?
2. Why do microbiologists wear laboratory coats? Did you confirm
that this is
3. Why is it necessary to wear clean, protective clothing when
caring for a
4. Why should hair be kept clean and out of the way when caring
5. How can the number of microorganisms in the environment be
6. When and why is hand washing important in patient care?
7. How can those who care for patients avoid spreading
Exercise 20.1 and 20.2
1. Differentiate the microscopic morphology of staphylococci and
streptococci as seen by Gram stain.
2. What are the two types of staphylococcal coagulase?
3. What is protein A? Describe one method of detecting it.
4. What properties ofS. aureusdistinguish it
5. How isS.
saprophyticusdistinguished fromS. epidermidis?
6. From what specimen type wouldS. saprophyticusmost
likely be isolated?
7. What is a nosocomial infection? Who acquires it? Why?
8. Why are staphylococcal infections frequent among hospital
9. Describe the role played byS. aureusin human
Page 1 of 2
FromLaboratory Manual &
Workbook in Microbiology Applications to Patient Care (9th ed.). By
Josephine A. Morello, Helen Eckel Mizer, and Paul A. Granato
Copyright © 2006 The McGraw-
Hill Companies, Inc. Reprinted with permission of The McGraw-Hill
1. What is the bacteriologic standard for potable water?
2. Why is bacteriologic analysis of water directed at recognition
and enterococci rather than isolation of pathogenic bacteria?
3. Define presumptive, confirmed, and completed tests of water.
4. Why must positive presumptive tests of water be confirmed?
5. In the Most Probable Number table, what is the significance of
confidence limits in relation to the MPN index/100 ml?
6. What is the public health significance of coliform-contaminated
7. List at least three waterborne infectious diseases.