A linear piece of DNA is cleaved with the restriction enzymes HindIII and SmaI, each alone and in combination, to yield the fragments of the size shown below:
HindIII: 1.0, 2.5, and 7.5 kb
SmaI: 5.5 kb
HindIII and SmaI: 1.0, 2.5, 3.0, and 4.5 kb
Draw a restriction map for the linear piece of DNA, showing all cut sites, labeled with the appropriate restriction enzyme, and noting the size of each fragment.
How long was the whole original linear fragment before cutting?