I have three questions about mutations in transcription/translation sate. 1. A mutation that makes the A-site of the ribosome about 40% less likely to bind to an amino-acyl tRNA
2. A mutation that changes a Threonine (Thr) tRNA anticodon from 3’UGC’5 to 3’UGG’5.
3. A mutation that allows sigma to bind more tightly to the promoter of the gene coding for initiation factor protein. (remember that initiation factor proteins help the small ribosomal subunit bind to an mRNA)
For each number, Can you list how would the mutation affect? thank you.